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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and therefore could contribute to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in advertising mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants were described but in depth nucleotide sequence information will not be but out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and it is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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